Resin In Ion Exchange Chromatography

Lanlang ® CS-1 Strong acid cation resin in calcium form Used for glucose and fructose separation
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Product Details

Product description:

CS-1 is a chromatogram resin with special particle size range. Lanlang ® CS-1 is a calcium type resin. Mainly used to separate and purify of glucose and fructose, the purity of fructose could improve from 42% to 80%-95%.

Physical and Chemical Properties:

Items

Index

Appearance

Yellow to Brown Beads

Ionic Form

Ca

ParticleSize Range %

(0.20~0.40mm)≥ 90.0

Operating Temperature ℃

~60

Temperature Limited ℃

120

Buffer Conditions:

The choice of buffer system, pH, additives and salt concentration all have a direct effect on the success of your IEX experiment. Buffer scouting is frequently required to find the optimal pH for solubility and adsorption to the IEX resin. When screening resins and buffer conditions, keep the following in mind:

  • Keep the pH of any protein purification or storage buffer 0.5 to 1 pH units above or below its pI to promote solubility. A protein’s pI is the point at which it has no net charge; it is likely to be unstable, less reactive, and least soluble at that pH. It’s a simple concept, but may not be the first thing that comes to mind if your protein begins crashing out of solution during buffer exchange.

  • Use a buffer concentration sufficient to maintain buffering capacity, typically 25 to 100 mM.

  • Pay attention to the ionic strength of the starting material and wash buffers, as the affinity of the protein for the column decreases as ionic strength increases due to salt concentration. There are more modern salt-tolerant ion exchangers that can help overcome this issue if you have to work with buffers at higher ionic strength (these will be highlighted in a later article).

  • If loading a small volume of protein onto an IEX column, dilute the protein solution with the starting buffer, which will assure that conditions are ideal for binding.

  • Slower flow-rates during column loading and elution increases the interaction time between the protein and the exchange resin, promoting specific binding interactions during loading.

Further information:

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