Rapid and efficient separation of simple and complex carbohydrates by high performance Ion-exchange chromatography resin is obtained using a quarternized alkylamine sorbent made of chemically modified nonporous and monodisperse highly cross-linked styrene copolymerparticles having a diameter of 2.5 μm and a surface area of 3 m2 g−1. In this technique there are many advantages of using short columns instead of those currently used in high performance anion-exchange chromatography in terms of separation time.
On the other hand, Ion exchange chromatography resin in the lithium form have been used for similar analyses using 90% ethanol in water eluents. The use of 4 and 8% cross-linked cation-exchange resins in the calcium or silverform have been used to provide a rapid separation of oligosaccharides. It is possible to obtain a total analysis of material applied to the column and the use of water as the only eluent